Cristiano Ronaldo’s hopes of winning the European Golden Boot as Europe’s top scorer suffered a blow as he failed to score as champions Juventus were beaten by Cagliari on Wednesday.

Ronaldo, 35, has set several records for Juventus this season becoming the first Juve player to score more than 25 goals in over half a century and his 31 Serie A goals this campaign is one short of Juve’s all-time record of goals for a single season set by Felice Borel in 1933-34.

The Portuguese forward, however, found himself unable to breach Cagliari’s backline on the night and ended the game on a frustrated note as he fell further behind in the race for Europe’s top scorer.

Luca Gagliano’s close-range finish and Giovanni Simeone’s fierce shot secured the win for the hosts.

Ronaldo trails Lazio striker Ciro Immobile by four goals in the battle for Europe’s Golden Shoe.

Immobile was on target with his 35th goal of the season as Lazio beat Brescia 2-0 on Wednesday.

Immobile’s goal means he is the outright leading scorer in Europe ahead of Bayern Munich’s Robert Lewandowski who finished the Bundesliga season with 34 goals.

With one game of the Serie A season remaining, it is unlikely that Ronaldo can catch Immobile who could also end the season with a new record for the most goals in a single Serie A season.

The Italian marksman is just one goal away from equalling the Serie A record for most goals in a season set by Juventus’ Gonzalo Higuain (36) for Napoli in the 2015-16 campaign.