Huddersfield Town are the first Premier League casualty of the season in the Carabao following the 2-0 loss the Terriers suffered at the hands of English Championship outfit Stoke City in their second round clash on Tuesday night.
Much maligned striker Saido Berahino scored the opening goal on 52 minutes for his first ever strike since his move in January 2017 before an own goal from Juninho Bacuna wrapped up the result for the Potters who are through to the third round.
Premier League newcomers Cardiff City also lost to lower league opposition in the wake of their 3-1 home defeat to Championship club Norwich City.
In the only Premier League affair on Tuesday, Charlie Austin scored in the 88th minute to secure a 1-0 win at the home of Brighton and Hove Albion.
West Ham United also joined Southampton and Stoke City in the third round after a 3-1 victory over 10-man AFC Wimbledon in the London Derby clash.
Joe Pigott gave the hosts a first half lead before AFC Wimbledon defender Rodney MacDonald was sent off for two yellow cards.
West Ham United made the numerical advantage against the League One outfit count in the second half courtesy goals from defenders Issa Diop and Angelo Ogbonna as well as Mexico striker Javier Hernandez.
Wolverhampton Wanderers scored two second half goals through Leo Bonatini and Helder Costa (penalty) to seal a third round spot with a 2-0 win at the home of Championship club Sheffield Wednesday.
Crystal Palace, Bournemouth and Fulham recorded respective second round victories as well.
Palace won 1-0 at Welsh side Swansea City courtesy a strike from Danish striker Andrew Soerloth, while French striker Aboulaye Kamara scored two goals either side of halftime to seal a 2-0 win over League Two club Exeter City at the Craven Cottage.
South Coast outfit Bournemouth also got the better of another League Two side following the 3-0 win over MK Dons at the Vitality Stadium through goals from Lys Mousset, Ryan Fraser and Jordon Ibe.